LOCUS       40924_31625       12809 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pMpGWB234 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12809)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T.
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha
  JOURNAL   PLoS ONE 10 (9), E0138876 (2015)
  PUBMED    26406247
REFERENCE   2  (bases 1 to 12809)
  AUTHORS   Nishihama R, Ishizaki K, Kohchi T.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUN-2015) Contact:Ryuichi Nishihama Kyoto University, 
            Graduate School of Biostudies; Kitashirakawa-oiwake-cho, Sakyo-ku, 
            Kyoto, Kyoto 606-8502, Japan URL :http://www.lif.kyoto-u.ac.jp/e/
REFERENCE   3  (bases 1 to 12809)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12809)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2015"; volume: "10"; issue: "9"; pages: "E0138876"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (01-JUN-2015) Contact:Ryuichi Nishihama Kyoto University, Graduate 
            School of Biostudies"; volume: " Kitashirakawa-oiwake-cho, Sakyo-ku,
            Kyoto, Kyoto 606-8502, Japan URL :http"; pages: 
            "//www.lif.kyoto-u.ac.jp/e"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12809
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      316..1519
                     /label=MpHSP17.8A1 promoter
                     /note="MpHSP17.8A1 promoter"
                     /regulatory_class="promoter"
     protein_bind    1532..1656
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1693..1723
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             1777..2433
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             2778..3080
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(3124..3248)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3259..4134
                     /codon_start=1
                     /product="ligand binding domain of the glucocorticoid
                     receptor"
                     /label=ligand binding domain of the glucocorticoid rec
                     /note="region_name: GR; glucocorticoid receptor, a member
                     of the nuclear receptor superfamily"
                     /protein_id="BAS49864.1"
                     /translation="MASEARKTKKKIKGIQQATAGVSQDTSENPNKTIVPAALPQLTPT
                     LVSLLEVIEPEVLYAGYDSSVPDSAWRIMTTLNMLGGRQVIAAVKWAKAIPGFRNLHLD
                     DQMTLLQYSWMFLMAFALGWRSYRQSSGNLLCFAPDLIINEQRMSLPCMYDQCKHMLFV
                     SSELQRLQVSYEEYLCMKTLLLLSSVPKEGLKSQELFDEIRMTYIKELGKAIVKREGNS
                     SQNWQRFYQLTKLLDSMHEVVENLLTYCFQTFLDKTMSIEFPEMLAEIITNQIPKYSNG
                     NIKKLLFHQK"
     terminator      4150..4402
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4436..4452)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4460..4476
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4484..4514)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4529..4550)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     polyA_signal    complement(4635..4807)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(4867..5397)
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     promoter        complement(5460..6136)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     misc_feature    complement(6331..6355)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6876..7664
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     rep_origin      7913..8501
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(8687..8827)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(9171..9365)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9434..10504)
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10936..11562)
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgggt ctagaccagc cattatagca actatgttaa aagtgacagt tttctctaat
      361 cgggaatctt gtccgtattg aaagttccct gttcagtgac tgagccgctt aataatattt
      421 tcagtttcga gtacgaggga gagattgaac aaattttggg gtttcagggc taaattctgc
      481 aggggcttta gttttgagtc gggtgaaact ttcctaagtc ttggcaatga actcacattc
      541 gccattgtac ctattaaatg acccaaacat ccttgacaac aaattgacaa gtacgggaac
      601 atgatcttca atatgaaatt taatatgtcc tttatgttgg ctttcctcat caatgattgg
      661 aaactcacat acaaagaaaa gcgtgatata ataaatcact agaatcttct ccgctttcta
      721 agacagtaat gatggatcca cgtgagtgtg aaacatcaat atcaacatcg gtaggagtag
      781 agaagtttct aaatcagaaa aagtgaatag acaagacaac ttactccttg aacgagaata
      841 ttcctcgaga aagagggaaa agagtaaaaa gagtaaaaag ccatggtaca gaatagccca
      901 ttccatggtt attgcagtca aaccgctacg ccagcaaaaa cggaacgaaa ctaaacccgt
      961 tccacaaatt gcgaaagatg gaatgtattc gccatgtata tttcgagatt attatgagac
     1021 ttccggtccc gggaagatgg cctgggttca ccgggatcag ccgaattccg acgtgatgga
     1081 gcacggccat gcatacttct agagaagtcg gccaccgtga aattcgacga cgagatggta
     1141 ctagaatcac cgggacagta cacacctcgt tcatggcctc gagcattcga tgtcgctctc
     1201 acctgcacag cctcgaggta tcgagagggt tctaggatgg accagcgatg tctcgaggaa
     1261 tttagaaaga tttcatgcgc tcgttgtgtg agagccaagg aagcacatta tggggcctat
     1321 aagtaccacg caagggcctg gggttcccat caatcaaatc agtgttcaga cgatttcctc
     1381 aagcgatcat tgcaccgtga tcagtgaagc acagacttca agcttttggc ttgcctactt
     1441 ccacgttccc atccgagatc gacaccgaat tgttgagttc tgtctgatct tcctgcagtc
     1501 gagagcgaag ttccaccatc tagagttatc aacaagtttg tacaaaaaag ctgaacgaga
     1561 aacgtaaaat gatataaata tcaatatatt aaattagatt ttgcataaaa aacagactac
     1621 ataatactgt aaaacacaac atatccagtc actatggcgg cccctcgagg ggccgcatta
     1681 ggcaccccag gctttacact ttatgcttcc ggctcgtata atgtgtggat tttgagttag
     1741 gatccggcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat
     1801 accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt
     1861 gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta
     1921 aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat
     1981 gctcatccgg aattccgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt
     2041 cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa
     2101 taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt
     2161 gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat
     2221 ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc
     2281 cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg
     2341 attcaggttc atcatgccgt ctgtgatggc ttccatgtcg gcagaatgct taatgaatta
     2401 caacagtact gcgatgagtg gcagggcggg gcgtaaacgc gtggatccgg cttactaaaa
     2461 gccagataac agtatgcgta tttgcgcgct gatttttgcg gtataagaat atatactgat
     2521 atgtataccc gaagtatgtc aaaaagaggt gtgctatgaa gcagcgtatt acagtgacag
     2581 ttgacagcga cagctatcag ttgctcaagg catatatgat gtcaatatct ccggtctggt
     2641 aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa
     2701 tcaggaaggg atggctgagg tcgcccggtt tattgaaatg aacggctctt ttgctgacga
     2761 gaacagggac tggtgaaatg cagtttaagg tttacaccta taaaagagag agccgttatc
     2821 gtctgtttgt ggatgtacag agtgatatta ttgacacgcc cgggcgacgg atggtgatcc
     2881 ccctggccag tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc
     2941 atatcgggga tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctccg
     3001 ttatcgggga agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta
     3061 acctgatgtt ctggggaata taaatgtcag gctcccttat acacagccag tctgcaggtc
     3121 gaccatagtg actggatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa
     3181 atctaattta atatattgat atttatatca ttttacgttt ctcgttcagc tttcttgtac
     3241 aaagtggttg ataacagcat ggctagtgaa gctcgaaaaa caaagaaaaa aatcaaaggg
     3301 attcagcaag ccactgcagg agtctcacaa gacacttcgg aaaatcctaa caaaacaata
     3361 gttcctgcag cattaccaca gctcacccct accttggtgt cactgctgga ggtgattgaa
     3421 cccgaggtgt tgtatgcagg atatgatagc tctgttccag attcagcatg gagaattatg
     3481 accacactca acatgttagg tgggcgtcaa gtgattgcag cagtgaaatg ggcaaaggcg
     3541 ataccaggct tcagaaactt acacctggat gaccaaatga ccctgctaca gtactcatgg
     3601 atgtttctca tggcatttgc cctgggttgg agatcataca gacaatcaag tggaaacctg
     3661 ctctgctttg ctcctgatct gattattaat gagcagagaa tgtctctacc ctgcatgtat
     3721 gaccaatgta aacacatgct gtttgtctcc tctgaattac aaagattgca ggtatcctat
     3781 gaagagtatc tctgtatgaa aaccttactg cttctctcct cagttcctaa ggaaggtctg
     3841 aagagccaag agttatttga tgagattcga atgacttata tcaaagagct aggaaaagcc
     3901 atcgtcaaaa gggaagggaa ctccagtcag aactggcaac ggttttacca actgacaaaa
     3961 cttctggact ccatgcatga ggtggttgag aatctcctta cctactgctt ccagacattt
     4021 ttggataaga ccatgagtat tgaattccca gagatgttag ctgaaatcat cactaatcag
     4081 ataccaaaat attcaaatgg aaatatcaag aagctgctgt ttcatcaaaa atagagctcg
     4141 aatttccccg atcgttcaaa catttggcaa taaagtttct taagattgaa tcctgttgcc
     4201 ggtcttgcga tgattatcat ataatttctg ttgaattacg ttaagcatgt aataattaac
     4261 atgtaatgca tgacgttatt tatgagatgg gtttttatga ttagagtccc gcaattatac
     4321 atttaatacg cgatagaaaa caaaatatag cgcgcaaact aggataaatt atcgcgcgcg
     4381 gtgtcatcta tgttactaga tcgggaattg gttccggaac caattcgtaa tcatggtcat
     4441 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     4501 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta ggctgaatta
     4561 ggcgcgccta tttctgaatt caacacattg cggacgtttt taatgtactg aattaacgcc
     4621 gaattaattc gggggatctg gattttagta ctggattttg gttttaggaa ttagatttta
     4681 ttgatagaag tattttacaa atacaaatac atactaaggg tttcttatat gctcaacaca
     4741 tgagcgaaac cctataggaa ccctaattcc cttatctggg aactactcac acattattat
     4801 ggagaaactc gagcttgtcg atcgactcta ggccgggaag ccgatctcgg cttgaacgaa
     4861 ttgttaggtg gcggtacttg ggtcgatatc aaagtgcatc acttcttccc gtatgcccaa
     4921 ctttgtatag agagccactg cgggatcgtc accgtaatct gcttgcacgt agatcacata
     4981 agcaccaagc gcgttggcct catgcttgag gagattgatg agcgcggtgg caatgccctg
     5041 cctccggtgc tcgccggaga ctgcgagatc atagatatag atctcactac gcggctgctc
     5101 aaacttgggc agaacgtaag ccgcgagagc gccaacaacc gcttcttggt cgaaggcagc
     5161 aagcgcgatg aatgtcttac tacggagcaa gttcccgagg taatcggagt ccggctgatg
     5221 ttgggagtag gtggctacgt ctccgaactc acgaccgaaa agatcaagag cagcccgcat
     5281 ggatttgact tggtcagggc cgagcctaca tgtgcgaatg atgcccatac ttgagccacc
     5341 taactttgtt ttagggcgac tgccctgctg cgtaacatcg ttgctgctgc gtaccatggt
     5401 cgatcgacag atctgcgaaa gctcgagaga gatagatttg tagagagaga ctggtgattt
     5461 cagcgtgtcc tctccaaatg aaatgaactt ccttatatag aggaagggtc ttgcgaagga
     5521 tagtgggatt gtgcgtcatc ccttacgtca gtggagatat cacatcaatc cacttgcttt
     5581 gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg gggtccatct
     5641 ttgggaccac tgtcggcaga ggcatcttga acgatagcct ttcctttatc gcaatgatgg
     5701 catttgtagg tgccaccttc cttttctact gtccttttga tgaagtgaca gatagctggg
     5761 caatggaatc cgaggaggtt tcccgatatt accctttgtt gaaaagtctc aatagccctt
     5821 tggtcttctg agactgtatc tttgatattc ttggagtaga cgagagtgtc gtgctccacc
     5881 atgttcacat caatccactt gctttgaaga cgtggttgga acgtcttctt tttccacgat
     5941 gctcctcgtg ggtgggggtc catctttggg accactgtcg gcagaggcat cttgaacgat
     6001 agcctttcct ttatcgcaat gatggcattt gtaggtgcca ccttcctttt ctactgtcct
     6061 tttgatgaag tgacagatag ctgggcaatg gaatccgagg aggtttcccg atattaccct
     6121 ttgttgaaaa gtctcaatag ccctttggtc ttctgagact gtatctttga tattcttgga
     6181 gtagacgaga gtgtcgtgct ccaccatgtt ggcaagctgc tctagccaat acgcaaaccg
     6241 aattcagaaa taaattcagc ctaattcggc gttaattcag tacattaaaa acgtccgcaa
     6301 tgtgttatta agttgtctaa gcgtcaattt gtttacacca caatatatcc tgccaccagc
     6361 cagccaacag ctccccgacc ggcagctcgg cacaaaatca ccactcgata caggcagccc
     6421 atcagtccgg gacggcgtca gcgggagagc cgttgtaagg cggcagactt tgctcatgtt
     6481 accgatgcta ttcggaagaa cggcaactaa gctgccgggt ttgaaacacg gatgatctcg
     6541 cggagggtag catgttgatt gtaacgatga cagagcgttg ctgcctgtga tcaattcggg
     6601 cacgaaccca gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc
     6661 gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg
     6721 ttttcatggc ttgttatgac tgtttttttg gggtacagtc tatgcctcgg gcatccaagc
     6781 agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc
     6841 agcagggcag tcgccctaaa acaaagttaa acatcatggg ggaagcggtg atcgccgaag
     6901 tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc
     6961 tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg
     7021 atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg
     7081 accttttgga aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca
     7141 ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat
     7201 ttggagaatg gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca
     7261 ttgatctggc tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag
     7321 cggcggagga actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa
     7381 ccttaacgct atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta
     7441 cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg
     7501 ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac
     7561 aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat
     7621 ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg caaataatgt ctagctagaa
     7681 attcgttcaa gccgacgccg cttcgcggcg cggcttaact caagcgttag atgcactaag
     7741 cacataattg ctcacagcca aactatcagg tcaagtctgc ttttattatt tttaagcgtg
     7801 cataataagc cctacacaaa ttgggagata tatcatgcat gaccaaaatc ccttaacgtg
     7861 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     7921 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     7981 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     8041 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     8101 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     8161 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     8221 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     8281 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     8341 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     8401 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     8461 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     8521 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     8581 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     8641 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt
     8701 ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct
     8761 gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat
     8821 ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     8881 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     8941 accgtcatca ccgaaacgcg cgaggcaggg tgccttgatg tgggcgccgg cggtcgagtg
     9001 gcgacggcgc ggcttgtccg cgccctggta gattgcctgg ccgtaggcca gccatttttg
     9061 agcggccagc ggccgcgata ggccgacgcg aagcggcggg gcgtagggag cgcagcgacc
     9121 gaagggtagg cgctttttgc agctcttcgg ctgtgcgctg gccagacagt tatgcacagg
     9181 ccaggcgggt tttaagagtt ttaataagtt ttaaagagtt ttaggcggaa aaatcgcctt
     9241 ttttctcttt tatatcagtc acttacatgt gtgaccggtt cccaatgtac ggctttgggt
     9301 tcccaatgta cgggttccgg ttcccaatgt acggctttgg gttcccaatg tacgtgctat
     9361 ccacaggaaa gagacctttt cgaccttttt cccctgctag ggcaatttgc cctagcatct
     9421 gctccgtaca ttaggaaccg gcggatgctt cgccctcgat caggttgcgg tagcgcatga
     9481 ctaggatcgg gccagcctgc cccgcctcct ccttcaaatc gtactccggc aggtcatttg
     9541 acccgatcag cttgcgcacg gtgaaacaga acttcttgaa ctctccggcg ctgccactgc
     9601 gttcgtagat cgtcttgaac aaccatctgg cttctgcctt gcctgcggcg cggcgtgcca
     9661 ggcggtagag aaaacggccg atgccgggat cgatcaaaaa gtaatcgggg tgaaccgtca
     9721 gcacgtccgg gttcttgcct tctgtgatct cgcggtacat ccaatcagct agctcgatct
     9781 cgatgtactc cggccgcccg gtttcgctct ttacgatctt gtagcggcta atcaaggctt
     9841 caccctcgga taccgtcacc aggcggccgt tcttggcctt cttcgtacgc tgcatggcaa
     9901 cgtgcgtggt gtttaaccga atgcaggttt ctaccaggtc gtctttctgc tttccgccat
     9961 cggctcgccg gcagaacttg agtacgtccg caacgtgtgg acggaacacg cggccgggct
    10021 tgtctccctt cccttcccgg tatcggttca tggattcggt tagatgggaa accgccatca
    10081 gtaccaggtc gtaatcccac acactggcca tgccggccgg ccctgcggaa acctctacgt
    10141 gcccgtctgg aagctcgtag cggatcacct cgccagctcg tcggtcacgc ttcgacagac
    10201 ggaaaacggc cacgtccatg atgctgcgac tatcgcgggt gcccacgtca tagagcatcg
    10261 gaacgaaaaa atctggttgc tcgtcgccct tgggcggctt cctaatcgac ggcgcaccgg
    10321 ctgccggcgg ttgccgggat tctttgcgga ttcgatcagc ggccgcttgc cacgattcac
    10381 cggggcgtgc ttctgcctcg atgcgttgcc gctgggcggc ctgcgcggcc ttcaacttct
    10441 ccaccaggtc atcacccagc gccgcgccga tttgtaccgg gccggatggt ttgcgaccgc
    10501 tcacgccgat tcctcgggct tgggggttcc agtgccattg cagggccggc agacaaccca
    10561 gccgcttacg cctggccaac cgcccgttcc tccacacatg gggcattcca cggcgtcggt
    10621 gcctggttgt tcttgatttt ccatgccgcc tcctttagcc gctaaaattc atctactcat
    10681 ttattcattt gctcatttac tctggtagct gcgcgatgta ttcagatagc agctcggtaa
    10741 tggtcttgcc ttggcgtacc gcgtacatct tcagcttggt gtgatcctcc gccggcaact
    10801 gaaagttgac ccgcttcatg gctggcgtgt ctgccaggct ggccaacgtt gcagccttgc
    10861 tgctgcgtgc gctcggacgg ccggcactta gcgtgtttgt gcttttgctc attttctctt
    10921 tacctcatta actcaaatga gttttgattt aatttcagcg gccagcgcct ggacctcgcg
    10981 ggcagcgtcg ccctcgggtt ctgattcaag aacggttgtg ccggcggcgg cagtgcctgg
    11041 gtagctcacg cgctgcgtga tacgggactc aagaatgggc agctcgtacc cggccagcgc
    11101 ctcggcaacc tcaccgccga tgcgcgtgcc tttgatcgcc cgcgacacga caaaggccgc
    11161 ttgtagcctt ccatccgtga cctcaatgcg ctgcttaacc agctccacca ggtcggcggt
    11221 ggcccatatg tcgtaagggc ttggctgcac cggaatcagc acgaagtcgg ctgccttgat
    11281 cgcggacaca gccaagtccg ccgcctgggg cgctccgtcg atcactacga agtcgcgccg
    11341 gccgatggcc ttcacgtcgc ggtcaatcgt cgggcggtcg atgccgacaa cggttagcgg
    11401 ttgatcttcc cgcacggccg cccaatcgcg ggcactgccc tggggatcgg aatcgactaa
    11461 cagaacatcg gccccggcga gttgcagggc gcgggctaga tgggttgcga tggtcgtctt
    11521 gcctgacccg cctttctggt taagtacagc gataaccttc atgcgttccc cttgcgtatt
    11581 tgtttattta ctcatcgcat catatacgca gcgaccgcat gacgcaagct gttttactca
    11641 aatacacatc acctttttag acggcggcgc tcggtttctt cagcggccaa gctggccggc
    11701 caggccgcca gcttggcatc agacaaaccg gccaggattt catgcagccg cacggttgag
    11761 acgtgcgcgg gcggctcgaa cacgtacccg gccgcgatca tctccgcctc gatctcttcg
    11821 gtaatgaaaa acggttcgtc ctggccgtcc tggtgcggtt tcatgcttgt tcctcttggc
    11881 gttcattctc ggcggccgcc agggcgtcgg cctcggtcaa tgcgtcctca cggaaggcac
    11941 cgcgccgcct ggcctcggtg ggcgtcactt cctcgctgcg ctcaagtgcg cggtacaggg
    12001 tcgagcgatg cacgccaagc agtgcagccg cctctttcac ggtgcggcct tcctggtcga
    12061 tcagctcgcg ggcgtgcgcg atctgtgccg gggtgagggt agggcggggg ccaaacttca
    12121 cgcctcgggc cttggcggcc tcgcgcccgc tccgggtgcg gtcgatgatt agggaacgct
    12181 cgaactcggc aatgccggcg aacacggtca acaccatgcg gccggccggc gtggtggtgt
    12241 cggcccacgg ctctgccagg ctacgcaggc ccgcgccggc ctcctggatg cgctcggcaa
    12301 tgtccagtag gtcgcgggtg ctgcgggcca ggcggtctag cctggtcact gtcacaacgt
    12361 cgccagggcg taggtggtca agcatcctgg ccagctccgg gcggtcgcgc ctggtgccgg
    12421 tgatcttctc ggaaaacagc ttggtgcagc cggccgcgtg cagttcggcc cgttggttgg
    12481 tcaagtcctg gtcgtcggtg ctgacgcggg catagcccag caggccagcg gcggcgctct
    12541 tgttcatggc gtaatgtctc cggttctagt cgcaagtatt ctactttatg cgactaaaac
    12601 acgcgacaag aaaacgccag gaaaagggca gggcggcagc ctgtcgcgta acttaggact
    12661 tgtgcgacat gtcgttttca gaagacggct gcactgaacg tcagaagccg actgcactat
    12721 agcagcggag gggttggatc aaagtacttt gatcccgagg ggaaccctgt ggttggcatg
    12781 cacatacaaa tggacgaacg gataaacct
//
